Back to Annual Meeting

Back to Annual Meeting

Abstract #159722

Ruel Michelin¹, Antony M. Kinyua, PhD², Yvonne L. Bronner, ScD³, Joseph Whittaker, PhD⁴, Ava Joubert, MD, DrPH(c)⁴, Frederick O. Oladeinde, PhD⁵, and Arthur Williams, PhD⁶. (1) School of Computer, Mathematical and Natural Sciences, Department of Biology, Morgan State University, 1700 East Cold Spring Lane, Baltimore, MD 21251-0001, 443-885-3540, ruemichel@hotmail.com, (2) School of Public Health and Policy, Center for Complementary and Alternative Medicine, Morgan State University, 1700 East Cold Spring Lane, Baltimore, MD 21251-0001, (3) School of Community Health and Policy, Morgan State University, Room 209, 4530 Portage Ave, Baltimore, MD 21251, (4) Morgan State University, 1700 E Cold Spring Lane, SCMNS, Baltimore, MD 21251, (5) School of Computer, Mathematical and Natural Sciences, Department of Chemistry, Morgan State University, 1700 East Cold Spring Lane, Baltimore, MD 21251-0001, (6) Biology, Morgan State University, 1700 E Cold Spring Lane, Spencer Hall, Room G12, Baltimore, MD 21251

Introduction: Cancers are increasingly becoming an economic burden on health care systems, especially of developing economies. High medical cost has resulted in a dependence on herbal and complimentary therapies. Here we investigated the effects of an extract containing lactones obtained from the recently identified Bacillus mojavensis strain nov. C14135 on MCF7 breast cancer cells. Materials/Methods: MCF7 breast adenocarcinoma cells cultured in RPMI 1640 HyQ® media supplemented with 10%FBS, Penn/Strep and Amphotericin B. Flasks incubated at 37oC, 5% CO2 and controlled RH. Cells grown to approx. 95% confluence, then treated with 200µl of .22µm-filtered extract. Control flasks remained untreated. Incubated flasks were checked at 12hr, 24hr and 48hr interval. Results: Microscopic analysis of cells revealed extensive cellular damage. Dye exclusion analysis employing Trypan blue revealed significant cell mortality resulting in a reduction in cell numbers. We employed Mitochondria Dehydrogenase (MDS) assay and Annexin-FITC-V Apoptosis analysis, which confirmed prior results. Conclusion: We report what appears to be possibly cellular shrinking and aggregation, followed by detachment from the substrate and eventual cellular destruction. The results appear to demonstrate that compounds within this fermented fraction are potentially catatonic to these breast cancer cells. This bioactivity of the extract appears to be unique and is possibly a result of innate compounds including Pyrrolo [1, 2-a] piperazine-3, 6-dione, identified following GC/MS fragmentation. Further experiments will ensue to identify, isolate and demonstrate the involvement and potential activity of the identified compounds. Analysis will include comparing results against currently used antineoplastic agents.

Learning Objectives:

• Audience will be exposed to potential novel cancer fighting compounds.
• Become exposed to the antineoplastic activity of metabolites fermented by a novel bacterium.
• Audience will be able to critically analyze the findings presented in this research.

Keywords: Breast Cancer, Public Health

Presenting author's disclosure statement:

Any relevant financial relationships? No
Any institutionally-contracted trials related to this submission?

I agree to comply with the American Public Health Association Conflict of Interest and Commercial Support Guidelines, and to disclose to the participants any off-label or experimental uses of a commercial product or service discussed in my presentation.