 |
The 130th Annual Meeting of APHA |
|
The 130th Annual Meeting of APHA |
Hui-Ju Tsai, Department of Human Genetics, University of Pittsburgh, A300 Crabtree Hall, Pittsburgh, PA 15261, (412) 687-8674, hut1@pitt.edu
Rationale: Type 2 diabetes is a major public health problem and a common disease that affects ~135 million people on a global scale. Although genome-wide scans have identified several chromosomal susceptibility regions, finding a causative gene has remained an enigma. A previous study has reported a novel gene, calpain-10, located on chromosome 2q37.3, as a putative susceptibility gene. Three single nucleotide polymorphisms (SNP43, SNP19, SNP63) were shown to be involved in increased risk of disease among Mexican Americans. In this study, we analyzed the association of these three polymorphisms with type 2 diabetes in Samoans. Methods: We computed allele frequencies for three SNPs, then tested if there was a significant difference between controls and cases by a contingency c2 test. Haplotype frequencies were estimated by the EM algorithm implemented in the programs EH, ZAPLO and PHASE. Linkage disequilibrium was evaluated for each pairwise comparison in cases and controls. We calculated the standardized disequilibrium coefficient and the delta-squared measure of disequilibrium for each pair of the SNPs. The association of each pairwise comparison was tested by Fisher’s exact test. For association analyses, we used a nonparametric test T5 statistic to test if haplotype frequencies were significantly different between cases and controls. Results/Conclusion: In our sample consisting of 96 controls and 176 cases, there is no significant evidence of association between cases and controls in allele frequencies, haplotype frequencies or haplotype combinations of three SNPs. Also, there is no evidence of linkage in the region of chromosome 2 containing these three SNPs.
Learning Objectives:
Keywords: Diabetes,
Presenting author's disclosure statement:
I do not have any significant financial interest/arrangement or affiliation with any organization/institution whose products or services are being discussed in this session.