222369 Different aspects of Bisphenol A(BPA) as endocrine disruptor chemical in Human Breast Cancer cells MCF-7

Wednesday, November 10, 2010

Miranda Lalloo , Env Health Science, NYMC, Valhalla, NY
Hong-Duck Kim , Env Health Science, NYMC, Valhalla, NY
Michael Shakarjian , Env Health Science, NYMC, Valhalla, NY
Bisphenol A (BPA) is a common constituent of plastics and a current public health concern. The aim of this study was to explore the effects of BPA exposure using the estrogen-responsive human breast cancer cell line, MCF-7. We exposed MCF-7 cells to BPA and measured several toxicologic endpoints, such as cell viability, proliferation, and apoptosis. BPA equivalently reduced MCF-7 cell viability and proliferation with an IC50 of ~20 ÁM. DNA fragmentation, an indicator of programmed cell death (apoptosis) was detected after 72 h exposure to 10 ÁM BPA. The effects of 17▀-estradiol (E2) differed from BPA. E2 caused less apoptosis and its antiproliferative effect varied as a function of glucose concentration in the medium. To define glucose effect on estrogen receptor (ER) and cytokine gene expression against BPA sensitivity, cells were grown in high glucose (4.5 g/L) and low glucose (1.0 g/L) medium and the relative mRNA levels of various cytokines were determined using PCR techniques. BPA treatment increased the relative mRNA of cytokines TGFα, TNFα and IL-6 mRNA fivefold. However relative mRNA of ERα and ER▀ remained unchanged in high or low glucose conditions. Conversely, Peroxisome Proliferator-Activated Receptor (PPAR) ▀ mRNA levels increased upon exposure to 10 ÁM of BPA under high glucose conditions. Our results indicate that while micromolar levels of PBA are directly toxic to estrogen-responsive cells, there are clear differences between the cellular responses to BPA and estrogens.

Learning Areas:
Basic medical science applied in public health
Chronic disease management and prevention
Conduct evaluation related to programs, research, and other areas of practice
Environmental health sciences
Public health biology
Public health or related research

Learning Objectives:
Assess

Presenting author's disclosure statement:

Qualified on the content I am responsible for because: I am MPH candidate in Shcool of Public Health at NYMC.
Any relevant financial relationships? No

I agree to comply with the American Public Health Association Conflict of Interest and Commercial Support Guidelines, and to disclose to the participants any off-label or experimental uses of a commercial product or service discussed in my presentation.