231559 Abundance and distribution of tetracycline resistance genes in freshwater aquaculture facilities

Monday, November 8, 2010 : 9:10 AM - 9:30 AM

Erin Seyfried, MS , Region 10, US Environmental Protection Agency, Seattle, WA
We investigated the occurrence and abundance of tetracycline resistance genes (tetR) in four aquaculture facilities with contrasting OTC treatment histories. End-point and real-time polymerase chain reaction methods were used to detect and quantify tetR genes in water, sediment and feed samples collected from the four aquaculture facilities. Effluent water samples had markedly higher tetR gene concentrations than did corresponding influent samples (3- to 50-fold higher). TetR concentrations in sedimentation pond samples were higher in control facilities than in treatment facilities (5- to 600-fold higher). Surprisingly, unmedicated feed contained substantial concentrations of all genes, but medicated feed had significantly higher tetR levels than unmedicated feed (1- to 40-fold higher). OTC concentrations were quantified for each of these matrices and were compared to the corresponding tetR concentrations. This study has important implications for the management of aquaculture activities and underscores the need to further evaluate potential environmental reservoirs of antibiotic resistance.

Learning Areas:
Environmental health sciences
Public health biology

Learning Objectives:
Be able to discuss the impacts of antibiotics use on aqauculture facilities and the environment

Presenting author's disclosure statement:

Qualified on the content I am responsible for because: I am qualified to present because my graduate degree research focused on antibiotic use at aquaculture facilties.
Any relevant financial relationships? No

I agree to comply with the American Public Health Association Conflict of Interest and Commercial Support Guidelines, and to disclose to the participants any off-label or experimental uses of a commercial product or service discussed in my presentation.