259205 Laboratory Preparedness: A multi-laboratory validation of non-typhoidal salmonella in drinking and surface water

Monday, October 29, 2012

Kaiser Shen, MS, MPH , ORISE Fellow- Water Security Division, Environmental Protection Agency, Washington, DC
The EPA Water Laboratory Alliance (WLA) is a nationwide laboratory network that provides the capability to analyze water samples during a contamination event. In 2011 the WLA completed a multi-laboratory validation of non-typhoidal Salmonella. The multi-laboratory validation study involved ten commercial, utility, and public health laboratories from eight states. The Study included the analysis of PBS, drinking water, and surface water samples spiked with S. typhimurium BioBall® spikes. The results of this study were used to assess method performance (i.e., recovery and precision) across multiple laboratories and matrices, compare effect of holding time (0 - 8 hours after sample spiking, compared to 30 ± 1 hours after sample spiking), assess reproducibility of results from analyses of “blind” samples collected from each of two sites per matrix (drinking water and surface water), and develop QC acceptance criteria. This Method is needed to address gaps that exist within standardized methods for isolation and analysis of biological agents. Ultimately, the validation of this protocol enhances laboratory capabilities, improving the ability of the Water Sector to respond to water contamination incidents.

Learning Areas:
Environmental health sciences
Public health biology

Learning Objectives:
To discuss the results of the non-typhoidal multi-laboratory validation study. To explain how this study was implemented in EPA's Full Scale Exercise.

Keywords: Environment, Pathogens

Presenting author's disclosure statement:

Qualified on the content I am responsible for because: I have been the co-lead in conducting biological method development studies for EPA's Water Security Division.
Any relevant financial relationships? No

I agree to comply with the American Public Health Association Conflict of Interest and Commercial Support Guidelines, and to disclose to the participants any off-label or experimental uses of a commercial product or service discussed in my presentation.