274945 Time-of-Flight Mass Spectrometry: A Simple but Versatile Analytical Tool for Identifying Toxicants

Tuesday, October 30, 2012 : 2:30 PM - 2:50 PM

Roy Gerona, PhD , Laboratory Medicine, University of California, San Francisco, San Francisco, CA
Current testing methods for environmental chemicals in people's bodies can be expanded to include more chemicals, a wider range of the human population, tissues other than blood and urine, and potential sources of exposure such as food. Combining these improvements with powerful new methods capable of identifying “non-targeted” chemicals (e.g. screening for unknown chemicals) will greatly expand our chemical testing capacity and our understanding of potential chemical contributors to disease. One new method is Liquid Chromatography- Time-of-Flight Mass Spectrometry (TOF LC-MS), which is capable of accurately matching the measured mass of a chemical with a molecular formula. With the use of appropriate chemical databases, this method can facilitate non-targeted analysis of environmental chemicals in environmental and biological samples allowing for identification of emerging environmental chemicals. This method is currently used for clinical and pharmaceutical testing, but its use in identifying environmental chemicals has been limited to date. The session will include a review of existing methods and an introduction to the next generation of biomonitoring techniques, with emphasis on the potential of TOF LC-MS for ‘non-targeted' testing

Learning Areas:
Environmental health sciences

Learning Objectives:
Explain the need for improved environmental chemical monitoring. Review and describe current analytical methods being used in environmental monitoring. Discuss the differences between targeted and non-targeted screening of chemicals in environmental and biological samples. Describe new methods for detecting emerging toxic chemicals in people and the environment.

Presenting author's disclosure statement:

Qualified on the content I am responsible for because: I am a clinical chemist specializing in the applications of LC-MS in clinical and environmental toxicology. I have set-up a mass spectrometry core laboratory for environmental biomonitoring. One of the unique focuses of the core laboratory is the implementation of non-targeted analysis using TOF LC-MS to discover novel targets for environmental biomonitoring. I have developed the analytical methods utilized in the collaborative work that will be presented by my collaborators in this session.
Any relevant financial relationships? No

I agree to comply with the American Public Health Association Conflict of Interest and Commercial Support Guidelines, and to disclose to the participants any off-label or experimental uses of a commercial product or service discussed in my presentation.