Breast cancer is the second most common cancer among women in the US (CDC, 2013). Studies suggest that cancer is diagnosed at later stages for Arab Americans and prevention efforts should be better understood (Arshad, 2011; Hirko et al., 2013). Cultural barriers and medical mistrust have been associated with breast cancer screening rates (Cohen & Azaiza, 2010; Thompson, et al, 2004). We examined the association between medical mistrust and breast cancer screening barriers – 1) environmental; 2) social; 3) body exposure; and 4) religious while controlling for demographic characteristics, employment, and religiosity. A heterogeneous sample of 196 women across different socioeconomic strata and Arab ancestry were recruited from a large health and social services agency and Mosques and administered a 15 minute paper and pencil questionnaire. A multiple regression analysis revealed a statistically significant association between the medical mistrust dimension of “suspicious of health care providers” and environment (β = .418, p=.000), social (β = .332, p=.001), body exposure (β = .226, p=.019), and religious (β = .208, p=.015) breast cancer screening barriers. Medical mistrust is positively associated with greater cultural barriers to breast cancer screening. These findings suggest that mistrust of medical care accentuates cultural barriers for Arab American women. This study illuminates the need to emphasize strategies that will target the medical care system and the cultural barriers to breast cancer screening among Arab American women.