Millions of vaccines are administered annually in the US. In ambulatory settings, most vaccines are manually recorded into Electronic Medical Record (EMR) systems using keyboard strokes. This is challenging at best, due to tiny type on vaccine vials and syringes. At worst, this time-consuming task can lead to errors and patient harm. A two-dimensional (2D) barcode holds several critical data elements (e.g., lot number, expiration date, product ID), and when scanned, can automate the vaccine recording process. 2D barcodes are now on most vaccine products, but scanning to record vaccines administered has not been widely adopted. The Centers for Disease Control and Prevention has partnered with various health facilities since 2011 to pilot the implementation and assess outcomes of vaccine 2D barcode scanning systems. Data were collected through a combination of de-identified EMR vaccine records, online surveys, time measurements, observations, and interviews. Scanning 2D barcodes resulted in significant time savings, improved vaccine record quality and completeness, and user satisfaction, but implementation challenges remained, including low scanning rates. With over 100 million vaccines likely administered annually in the US, even small improvements to vaccine record accuracy can have meaningful impact – a 1% improvement could impact a million or more vaccine records. However, benefits are only realized when scanning is actually used. To combat limited scanning use, we took a two-pronged approach: 1) maximize scanning rates through inclusion of adherence strategies, and 2) translate findings into formats encouraging longer-term scanning habits. Inclusion of three adherence strategies (signed commitment card, receiving scanning rates, and a combination of both) identified ways to improve scanning rates, compared to a training-only approach. Next, development of a six-step Implementation Guide created a decision-making and implementation planning resource. Steps include: deciding whether to adopt 2D scanning, implementation planning, training, evaluation, adjusting strategies to ensure use, and sustaining practices once implemented. Findings demonstrated both data accuracy (5-15%) and time savings (avg.21 seconds/vaccine) improvements. For one location these savings translated into 12+ additional vaccine appointments weekly. Translation and utilization of findings into practice are critical to affecting change for patients, practitioners, health organizations, and ultimately improving our nation’s health.