Cisgender women represent approximately 1 in 5 HIV diagnoses in New York City (NYC) of whom 61% are non-Latina Black and 27% are Latina. Because little is known about knowledge and attitudes surrounding PrEP in this population, we examined a population in NYC. In 2017, interviewer-administered (English/Spanish) street-intercept surveys were conducted in neighborhoods with high HIV-diagnosis rates among Black and/or Latina cisgender women who were NYC residents aged 18-64 years reporting recent sex with a man. We assessed PrEP awareness and recent use (past 6 months) and, among those aware, perceived benefit, comfort discussing with a provider, and agreement with attitudinal statements. Differences were tested by chi-square (p<0.05). Among respondents, 59.7% were non-Latina Black; mean age was 37 years; 51.0% had attended high school or less 51.6% earned <$20,000/year. PrEP awareness was 34.1% (126/370); recent use was 1.4% (5/361). Awareness was significantly higher among non-Latina Blacks (39.8%; 88/221) than Latinas (25.5%; 38/149) (p=0.004). Among those aware, 59.6% (56/94) did not feel they would benefit from PrEP, but 93.3% (98/105) would be comfortable discussing it with their provider. Most agreed that women should know more about PrEP (95.7%; 90/94) and users are taking control of their sexual health (91.7%; 88/96), although 64.0% (57/89) worried about potential adverse effects. Compared with Latina women, non-Latina Black women had higher PrEP awareness. Despite not perceiving personal benefit, women aware of PrEP expressed largely positive attitudes towards it, except for concern for potential adverse effects.