5075.0: Wednesday, November 15, 2000 - Board 4

Abstract #16458

Detection of Parasitic Infections by Immunological Techniques

Lutheria Peters, Abdalla Aldras, MSPH, ScD, and Adenike Bitto, MD, MPH, DrPH, CHES. East Stroudsburg University, P.O. Box 155, East Stroudsburg, PA 18301, 570-422-3375, abitto@po-box.esu.edu

Modern immunological techniques are now an essential tool in the diagnosis of infectious diseases. They are specific, sensitive, low cost and results can be obtained within a short period of time. This study will utilize several immunological techniques to examine three water-borne pathogens (Giardia lamblia, Cryptosporidium species and Microsporidium species). Several water samples will be tested for the presence of these parasites. Animals such as the beaver and muskrat will also be tested using the following techniques: immunofluorescence antibody test (IFA), Enzyme-linked immunosorbent assays (ElISA) and SDS-PAGE electrophoresis. All the tests will be done in conjunction with positive and negative controls. The results will show if the water samples are contaminated with one or more of these parasites (Giardia lamblia, Cryptosporidium, and Microsporidium). The density of these parasites in the contaminated water samples will also be determined. These water testing techniques are particularly important for small rural communities that may not have a municipal water or sewage treatment plant. Many rural communities rely on wells for their water supply. Thus, periodic water testing can be a useful adjunct for maintaining the public's health, and to help reduce health disparities between urban and rural areas.

Learning Objectives: At the end of this presentation participants will be able to describe the use of immunofluorescent techniques for detection of parasites such as giardia, cryptosporidium, and microsporidium in water samples

Keywords: Infectious Diseases, Water Test

Presenting author's disclosure statement:
Organization/institution whose products or services will be discussed: None
I do not have any significant financial interest/arrangement or affiliation with any organization/institution whose products or services are being discussed in this session.

The 128th Annual Meeting of APHA